antibodies targeting trmt6  (Proteintech)

Journal: BMC Gastroenterology

Article Title: Hypoxia-induced exosomal circNRIP1 activates cancer-associated fibroblasts to promote esophageal squamous cell carcinoma migration and invasion

doi: 10.1186/s12876-025-03978-w

Figure Lengend Snippet: circNRIP1 activates CAFs by binding to TRMT6. A Graphical representation of the molecular docking between circNRIP1 and the TRMT6 protein using HDock. B , C Western blot was conducted to validate the circNRIP1 pull-down products in fibroblasts. D The expressions of TRMT6 were determined by western blot and real-time PCR. E , F Transwell assays detected the migration of CAFs. G ELISA assays detected the secretion of IL-1β and TGF-β in CAFs. H Western blot detected the expression of α-SMA and COL1A1 in CAFs. (NC: fibroblasts without treatment, shcircNRIP1: fibroblasts co-cultured with shcircNRIP1 exosomes, TRMT6-OE: TRMT6-overexpressing fibroblasts, shcircNRIP1 + TRMT6-OE: TRMT6-overexpressing fibroblasts co-cultured with shcircNRIP1 exosomes.) Data represent mean ± S.D. of three independent experiments. * P < 0.05, ** P < 0.01, **** P < 0.0001

Article Snippet: Membranes were then treated with primary antibodies against ALIX (Proteintech, 12,422–1-AP, USA), TSG101 (Abcam, ab125011, USA), CD81 (Proteintech, 27,855–1-AP, USA), α-SMA (Proteintech, 14,395–1-AP, USA), COL1A1 (Proteintech,14,695–1-AP, USA), COL3A1 (Proteintech, 21,898–1-AP, USA), TRMT6 (Proteintech,16,727–1-AP,1:6000), GAPDH (Proteintech, 60,004–1-Ig, USA) at 4°C overnight.

Techniques: Binding Assay, Western Blot, Real-time Polymerase Chain Reaction, Migration, Enzyme-linked Immunosorbent Assay, Expressing, Cell Culture

Journal: BMC Gastroenterology

Article Title: Hypoxia-induced exosomal circNRIP1 activates cancer-associated fibroblasts to promote esophageal squamous cell carcinoma migration and invasion

doi: 10.1186/s12876-025-03978-w

Figure Lengend Snippet: Schematic representation of how hypoxia-derived exosomes from ESCC cells activate CAFs and promote tumor progression by delivering circNRIP1 to target TRMT6

Article Snippet: Membranes were then treated with primary antibodies against ALIX (Proteintech, 12,422–1-AP, USA), TSG101 (Abcam, ab125011, USA), CD81 (Proteintech, 27,855–1-AP, USA), α-SMA (Proteintech, 14,395–1-AP, USA), COL1A1 (Proteintech,14,695–1-AP, USA), COL3A1 (Proteintech, 21,898–1-AP, USA), TRMT6 (Proteintech,16,727–1-AP,1:6000), GAPDH (Proteintech, 60,004–1-Ig, USA) at 4°C overnight.

Techniques: Derivative Assay

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Quantifying the mRNA epitranscriptome reveals epitranscriptome signatures and roles in cancer

doi: 10.1007/s00018-025-05805-7

Figure Lengend Snippet: siRNA decreases the mRNA and protein levels of m 1 A regulatory enzymes in HeLa cells. (A) Relative expression changes of mRNA for TRMT10C , ALKBH3 , TRMT6 , TRMT61A , and TRMT6-61 A were measured by RT-qPCR. The x-axis represents the treatment groups, while the y-axis indicates the mRNA expression changes of m 1 A regulatory enzymes relative to the reference gene (GAPDH). (B) Protein level changes of TRMT10C, ALKBH3, TRMT6, TRMT61A, and TRMT6-61 A were assessed by Western blotting. Protein bands were quantified using ImageJ software. The x-axis represents the treatment groups, and the y-axis shows the changes in m1A regulatory enzymes relative to the reference gene (GAPDH). Each group was conducted with three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The protein samples were incubated with the corresponding primary antibodies: TRMT6 Polyclonal Antibody (Proteintech), TRMT10C Polyclonal Antibody (Proteintech), ALKBH3 Polyclonal Antibody (Proteintech), and TRMT61A Polyclonal Antibody (ORIGENE), followed by incubation with the respective secondary antibodies: Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (Proteintech) and Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (Proteintech).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Software

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Quantifying the mRNA epitranscriptome reveals epitranscriptome signatures and roles in cancer

doi: 10.1007/s00018-025-05805-7

Figure Lengend Snippet: Effects of m 1 A regulatory enzyme knockdown on cell viability. (A) CCK8 assay evaluating changes in cell proliferation following knockdown of m 1 A regulatory enzymes TRMT10C , ALKBH3 , TRMT6 , TRMT61A , and TRMT6-61 A at 6 h, 24 h, 48 h, and 72 h. The x-axis represents treatment duration, and the y-axis indicates cell viability. Each group consists of three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001. (B) Flow cytometry measurement of apoptosis rates after 72 h of knockdown of m 1 A regulatory enzymes TRMT10C , ALKBH3 , TRMT6 , TRMT61A , and TRMT6-61 A . Cell apoptosis percentages were quantified using GraphPad Prism software; the x-axis represents m 1 A regulatory enzymes, and the y-axis represents the percentage of apoptosis. Each group consists of three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The protein samples were incubated with the corresponding primary antibodies: TRMT6 Polyclonal Antibody (Proteintech), TRMT10C Polyclonal Antibody (Proteintech), ALKBH3 Polyclonal Antibody (Proteintech), and TRMT61A Polyclonal Antibody (ORIGENE), followed by incubation with the respective secondary antibodies: Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (Proteintech) and Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (Proteintech).

Techniques: Knockdown, CCK-8 Assay, Flow Cytometry, Software

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Quantifying the mRNA epitranscriptome reveals epitranscriptome signatures and roles in cancer

doi: 10.1007/s00018-025-05805-7

Figure Lengend Snippet: Transcriptomic and proteomic analysis of TRMT6-61 A treated HeLa cells: Transcriptome: (A) Top 20 KEGG enrichment bubble chart. Proteome: (B) Top 25 connectivity protein interaction network diagram. Combined transcriptomic and proteomic analysis: (C) Bar chart of the top 30 KEGG (GSEA) pathways shared across different omics

Article Snippet: The protein samples were incubated with the corresponding primary antibodies: TRMT6 Polyclonal Antibody (Proteintech), TRMT10C Polyclonal Antibody (Proteintech), ALKBH3 Polyclonal Antibody (Proteintech), and TRMT61A Polyclonal Antibody (ORIGENE), followed by incubation with the respective secondary antibodies: Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (Proteintech) and Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (Proteintech).

Techniques: